Activity of Oleic Acid on Biofilm Formation of S. aureus

Oleic acid is a naturally occurring fatty acid in animal and vegetable oils and has been shown to have a wide variety of pharmacological effects. It aimed to investigate the efficacy of oleic acid on the adhesion and invasion of S. aureus to the host cell and biofilm production. The standard S. aureus strain (ATCC 25923) was used in the experiments. Cytotoxicity tests of oleic acid were performed in the Vero cell line. Bacterial adhesion and invasion rates and activities on slime formation in cells treated with oleic acid were evaluated compared to the control group. Slime formation tests were evaluated phenotypically on Congo red agar. In the study, it was determined that oleic acid was effective in both cellular adhesion and invasion in terms of colony number in cell cultures treated with 0.156 µg/ml concentration of oleic acid. In addition, it was determined that slime production was significantly inhibited in bacterial cultures treated with oleic acid. Oleic acid prevents cells from attaching to bacteria and has an inhibitory effect on the virulence of bacteria. This activity of oleic acid may be due to its modulatory effect on cellular processes, and its bacterial virulence may be related to its effect on bacterial metabolism.