双功能
生物传感器
光电流
硫堇
材料科学
纳米技术
化学
电化学
光电子学
生物化学
电极
物理化学
催化作用
作者
Qingqing Zhang,Suying Liu,Hejie Zheng,Huan Wang,G.H. Luo,Xiaohua Zhang,Cuicui Du,Jinhua Chen
标识
DOI:10.1016/j.cej.2023.146592
摘要
Abnormal protein tyrosine kinase (PTK) expression is associated with various diseases and thus it is of great significance in analysis of PTK activity via a reliable method. Herein, magnetic photoactive Fe3O4@Sb2S3 nanospheres and bifunctional thionine-embedded metal organic frameworks (MOFs)-derived nitrogen-doped porous carbon (NPC@Thi) nanopolyhedra were successfully synthesized for constructing a highly sensitive magnetic-assisted homogeneous photoelectrochemical (PEC)–electrochemical (EC) dual-mode biosensing platform for the detection of PTK (c-Src) activity. The Fe3O4@Sb2S3 nanospheres with good magnetic and photoactive properties assisted adequate biological recognition in a homogeneous system, simplified sample separation and facilitated sensor construction. After c-Src-mediated specific recognition, bifunctional NPC@Thi nanopolyhedra were connected to Fe3O4@Sb2S3 nanospheres. NPC@Thi not only enhanced the photocurrent of Fe3O4@Sb2S3 but also acted as the electrochemical probe. Thus, highly sensitive and reliable dual “signal-on” assay of c-Src activity with wide linear response ranges and low detection limits was achieved by the successful cooperation of PEC (10 fM to 10 nM, 7.2 fM) and EC (5 fM to 10 nM, 3.8 fM) biosensing strategies. The proposed homogeneous PEC–EC dual-mode biosensing platform is prospective to be extended to the analysis of other PTK activity and the discovery of PTK-targeted drug.
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