木质部
韧皮部
维管形成层
木质素
血管组织
次生生长
次生细胞壁
植物
生物
形成层
维管束
植物茎
细胞生物学
转录因子
生物物理学
化学
基因
生物化学
作者
Chengwei Song,Yayu Guo,Weiwei Shen,Xiaomin Yao,Huimin Xu,Yuanyuan Zhao,Ruili Li,Jinxing Lin
出处
期刊:Plant Physiology
[Oxford University Press]
日期:2023-03-18
卷期号:192 (2): 1046-1062
被引量:4
标识
DOI:10.1093/plphys/kiad152
摘要
Abstract Secondary growth in woody plants generates new cells and tissues via the activity of the vascular cambium and drives the radial expansion of stems and roots. It is regulated by a series of endogenous factors, especially transcription factors. Here, we cloned the basic helix–loop–helix (bHLH) transcription factor gene UNFERTILIZED EMBRYO SAC12 (UNE12) from poplar (Populus alba × Populus glandulosa Uyeki) and used biochemical, molecular, and cytological assays to investigate the biological functions and regulatory mechanism of PagUNE12. PagUNE12 mainly localized in the nucleus and possessed transcriptional activation activity. It was widely expressed in vascular tissues, including primary phloem and xylem and secondary phloem and xylem. Poplar plants overexpressing PagUNE12 showed significantly reduced plant height, shorter internodes, and curled leaves compared with wild-type plants. Optical microscopy and transmission electron microscopy revealed that overexpressing PagUNE12 promoted secondary xylem development, with thicker secondary cell walls than wild-type poplar. Fourier transform infrared spectroscopy, confocal Raman microscopy, and 2D Heteronuclear Single Quantum Correlation analysis indicated that these plants also had increased lignin contents, with a lower relative abundance of syringyl lignin units and a higher relative abundance of guaiacyl lignin units. Therefore, overexpressing PagUNE12 promoted secondary xylem development and increased the lignin contents of secondary xylem in poplar, suggesting that this gene could be used to improve wood quality in the future.
科研通智能强力驱动
Strongly Powered by AbleSci AI