葡萄糖计
底漆(化妆品)
DNA
基因
溶解
人类免疫缺陷病毒(HIV)
赫拉
结合
化学
分子生物学
生物
生物化学
病毒学
细胞
数学分析
数学
有机化学
糖尿病
内分泌学
作者
Yayun An,Ailing Kan,Aimei Ouyang,Nan Zhang,Wei Jiang
标识
DOI:10.1016/j.snb.2023.134284
摘要
Sensitive and convenient detection of HIV gene fragment is essential for the screening of acquired immunodeficiency syndrome (AIDS), as well as for timely medical intervention and effective management of the disease. Here, we present a cascade amplification strategy for sensitive and convenient detection of HIV gene fragment utilizing a personal glucose meter. Two amplification reactions, polymerization nicking reaction and cross primer exchange reaction, were cascaded to generate copious long single-strand DNA (ssDNA). These ssDNA facilitated the recruitment of DNA-invertase conjugate that catalyzed the hydrolysis of sucrose, generating a signal that could be detected by a personal glucose meter. As a proof of concept, a mock fragment of HIV gene was employed as the target, which could be detected with picomolar sensitivity. Moreover, the proposed strategy stayed robust in 10% diluted human serum and Hela cell lysate, suggesting its potential utility for detecting HIV gene fragments in real-world diagnostic scenarios.
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