DNA损伤
细胞凋亡
信号转导
回转器
基因表达
电离辐射
生物
基因
辐照
细胞生物学
DNA修复
细胞周期
成纤维细胞
分子生物学
化学
遗传学
DNA
细胞培养
物理
核物理学
作者
Paola Malatesta,Konstantinos Kyriakidis,Megumi Hada,Hiroko Ikeda,Akio Takahashi,Premkumar B. Saganti,Alexandros G. Georgakilas,Ioannis Michalopoulos
出处
期刊:Biomolecules
[MDPI AG]
日期:2024-01-10
卷期号:14 (1): 88-88
摘要
During future space missions, astronauts will be exposed to cosmic radiation and microgravity (μG), which are known to be health risk factors. To examine the differentially expressed genes (DEG) and their prevalent biological processes and pathways as a response to these two risk factors simultaneously, 1BR-hTERT human fibroblast cells were cultured under 1 gravity (1G) or simulated μG for 48 h in total and collected at 0 (sham irradiated), 3 or 24 h after 1 Gy of X-ray or Carbon-ion (C-ion) irradiation. A three-dimensional clinostat was used for the simulation of μG and the simultaneous radiation exposure of the samples. The RNA-seq method was used to produce lists of differentially expressed genes between different environmental conditions. Over-representation analyses were performed and the enriched biological pathways and targeting transcription factors were identified. Comparing sham-irradiated cells under simulated μG and 1G conditions, terms related to response to oxygen levels and muscle contraction were identified. After irradiation with X-rays or C-ions under 1G, identified DEGs were found to be involved in DNA damage repair, signal transduction by p53 class mediator, cell cycle arrest and apoptosis pathways. The same enriched pathways emerged when cells were irradiated under simulated μG condition. Nevertheless, the combined effect attenuated the transcriptional response to irradiation which may pose a subtle risk in space flights.
科研通智能强力驱动
Strongly Powered by AbleSci AI