DNA Origami: Synthesis and Self‐Assembly

Abstract DNA origami is an emerging technology for designing defined two‐ and three‐dimensional (2D and 3D) DNA nanostructures. Here, we report an introductory practical guide with step‐by‐step experimental details for the design and synthesis of origami structures, and their size expansion in 1D and 2D space by means of self‐assembly. Curr. Protoc. Nucleic Acid Chem . 48:12.9.1‐12.9.18. © 2012 by John Wiley & Sons, Inc.