Behavioral study of sleep deprivation model in caffeine and light induced zebrafish

Objective: To compare the differences in behavior of sleep deprivation models in caffeine \nand light induced zebrafish. Methods: A total of 72 zebrafish larvae,were selected 5 d after \nfertilization and randomly divided into blank control group, caffeine and light group, with \n24 larvae in each group. The blank control group was given normal breeding for 24 h, while \non the basis of treatment in the blank control group, the caffeine group was exposed to 200 \nμmol/L caffeine in water and the light group to 200 lux light conditions continuously for 24 \nh. Results: The pigmentation of zebrafish head in the light group and the caffeine group was \nslightly shallow with edema of yolk sac. The activity time of the light group and the caffeine \ngroup increased compared with the blank control group (P < 0.01, P < 0.05), but there was \nno significiant difference in activity time between the light group and the caffeine group (P > \n0.05), The small movement time of the light group was significantly more than the caffeine \ngroup (P < 0.01), while the medium movement time of the caffeine group was more than light \ngroup (P < 0.05). Conclusion: Both light and caffeine can induce zebrafish sleep deprivation \nmodel effectively, and have effects on zebrafish phenotype to a certain degree, but there are \ndifferences in motor form between the two kinds of sleep-deprivation models.