Coordination with zirconium: A facile approach to improve the mechanical properties and thermostability of gelatin hydrogel

The poor mechanical property and thermostability restricted applications of gelatin hydrogel. Herein, a facile and inexpensive approach of immerging cooling induced gelatin hydrogels into Zr(SO4)2 dilute solution was applied to overcome these shortages. After this treatment, the micropores in hydrogel decreased to tens of microns while the water content slightly decreased. XPS results revealed that the coordination bonds formed between amino or carboxyl groups of gelatins and Zr4+. After immerging in 0.06 M Zr4+ solution, mechanical tests showed that the elastic modulus, compressive modulus and compressive strength of hydrogel were about 400, 1192 and 476 kPa, respectively, which were approximate 100, 11 and 5 times larger than those of pure gelatin. The DSC data indicated that the thermoreversible temperature of triple helix structure in gelatin was improved from about 30 °C to 55 °C. More importantly, the rheological temperature sweep test revealed that hydrogels with 0.06 M Zr4+ treatment can maintain the hydrogel state without melting even at 80 °C. CCK-8 tests and Calcein-AM/PI double-stain experiments demonstrated Zr4+ coordination was non-cytotoxic. These promising data indicated this nontoxic method was efficient and had potential to fabricate gelatin related materials for further application.