基因传递
遗传增强
生物
向性
病毒载体
体内
转染
转导(生物物理学)
基因靶向
基因治疗载体
体外
病毒学
载体(分子生物学)
细胞生物学
基因
病毒
遗传学
重组DNA
生物物理学
作者
Franz Scherer,Martina Anton,Ulrike Schillinger,Julia Henke,Christian Bergemann,Achim Krüger,Bernd Gänsbacher,Christian Plank
出处
期刊:Gene Therapy
[Springer Nature]
日期:2002-01-01
卷期号:9 (2): 102-109
被引量:848
标识
DOI:10.1038/sj.gt.3301624
摘要
Low efficiencies of nonviral gene vectors, the receptor-dependent host tropism of adenoviral or low titers of retroviral vectors limit their utility in gene therapy. To overcome these deficiencies, we associated gene vectors with superparamagnetic nanoparticles and targeted gene delivery by application of a magnetic field. This potentiated the efficacy of any vector up to several hundred-fold, allowed reduction of the duration of gene delivery to minutes, extended the host tropism of adenoviral vectors to nonpermissive cells and compensated for low retroviral titer. More importantly, the high transduction efficiency observed in vitro was reproduced in vivo with magnetic field-guided local transfection in the gastrointestinal tract and in blood vessels. Magnetofection provides a novel tool for high throughput gene screening in vitro and can help to overcome fundamental limitations to gene therapy in vivo.
科研通智能强力驱动
Strongly Powered by AbleSci AI