外域
化学
受体
生物物理学
Spike(软件开发)
穗蛋白
细胞生物学
病毒学
生物
2019年冠状病毒病(COVID-19)
生物化学
医学
计算机科学
软件工程
疾病
病理
传染病(医学专业)
作者
Robert J. Edwards,Katayoun Mansouri,Victoria Stalls,Kartik Manne,Brian Watts,Rob Parks,Katarzyna Janowska,S. Gobeil,Megan Kopp,Dapeng Li,Xiaozhi Lu,Zekun Mu,Margaret Deyton,Thomas H. Oguin,Jordan Sprenz,Wilton B. Williams,Kevin O. Saunders,David C. Montefiori,Gregory D. Sempowski,Rory Henderson,S. Munir Alam,Barton F. Haynes,Priyamvada Acharya
标识
DOI:10.1038/s41594-020-00547-5
摘要
The SARS-CoV-2 spike (S) protein, a primary target for COVID-19 vaccine development, presents its receptor binding domain in two conformations, the receptor-accessible ‘up’ or receptor-inaccessible ‘down’ states. Here we report that the commonly used stabilized S ectodomain construct ‘2P’ is sensitive to cold temperatures, and this cold sensitivity is abrogated in a ‘down’ state-stabilized ectodomain. Our findings will impact structural, functional and vaccine studies that use the SARS-CoV-2 S ectodomain. The SARS-CoV-2 spike ectodomain is destabilized by cold temperature storage, an effect that can be reversed by incubation at 37 °C or by stabilizing its conformation in the ‘down’ state.
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