机械转化
自愈水凝胶
细胞生物学
配体(生物化学)
生物医学工程
组织工程
干细胞
材料科学
纳米技术
焦点粘着
细胞粘附
细胞外基质
生物物理学
再生医学
机械生物学
受体
化学
生物
医学
高分子化学
生物化学
作者
Man Zhang,Qian Sun,Yiling Liu,Zhiqin Chu,Leixiao Yu,Yong Hou,Heemin Kang,Qiang Wei,Weifeng Zhao,Joachim P. Spatz,Changsheng Zhao,Elisabetta Ada Cavalcanti‐Adam
出处
期刊:Biomaterials
[Elsevier]
日期:2021-01-01
卷期号:268: 120543-120543
被引量:47
标识
DOI:10.1016/j.biomaterials.2020.120543
摘要
Hydrogels with tunable mechanical properties have provided a tremendous opportunity to regulate stem cell differentiation. Hydrogels with osteoid (about 30–40 kPa) or higher stiffness are usually required to induce the osteogenic differentiation of mesenchymal stem cells (MSCs). It is yet difficult to achieve the same differentiation on very soft hydrogels, because of low environmental mechanical stimuli and restricted cellular mechanotransduction. Here, we modulate cellular spatial sensing of integrin-adhesive ligands via quasi-hexagonally arranged nanopatterns to promote cell mechanosensing on hydrogels having low stiffness (about 3 kPa). The increased interligand spacing has been shown to regulate actomyosin force loading to recruit extra integrins on soft hydrogels. It therefore activates mechanotransduction and promotes the osteogenic differentiation of MSCs on soft hydrogels to the level comparable with the one observed on osteoid stiffness. Our work opens up new possibilities for the design of biomaterials and tissue scaffolds for regenerative therapeutics.
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