Trichromatic-emission and dual-ratio semiconducting polymer dots as fluorescent probe for simultaneous quantification of Cu2+and pHin vitroandin vivo

Novel dual-ratio semiconducting polymer dots (Pdots) with trichromatic-emission in the red, green and blue primary-color regions, are designed as lysosome-targeting nanoprobes for the simultaneous detection and multicolor imaging of pH and Cu²⁺ in HeLa cells and zebrafish without cross-interference.