Optimization of ultrasonic-assisted extraction technology of β-Glucan from barleys

Barleys were taken as raw materials and the ultrasonic-assisted extraction technology was used to extract β-Glucan. The single factor experiment and quadratic orthogonal Box -Benhnken regression combination design were adopted to study the effects of such factors as ultrasonic power, liquid -solid, extraction temperature, pH value and extraction time on β-Glucan yield. The optimal technological condition of β-Glucan extraction from barleys by ultrasonic was determined, then extraction regression mathematical model was established. The order of factors that influence the β-Glucan yield within the experimental range was as follows: ultrasonic power pH value extraction temperature extraction time. The optimal technological parameters of β-Glucan extraction from barleys should be as follows: extraction temperature 60℃, extraction time 20 minutes, ultrasonic frequency 20 kHz, power 400 W, liquid-solid 20:1(v/m), pH value 10, 2 times. And such conditions, the β -Glucan yield after extraction three times is 3.65% . and the established regression mathematical model can predict the β-Glucan yield accurately