Acute Toxicity Experience to Guppy with Ciprofloxacin HCI,Enrofloxacin and Norfloxacin

Under the room temperature,static water conditions,the experiment took advantage of ciprofloxacin HCI,enrofloxacin and norfloxacin.And it studied the acute toxicity experience to the guppy with the experimental methods of comparative analysis and toxicology.This experiment will firstly set 5 teams of concentration gradient for the 3 drugs.And it is ciprofloxacin HCI 300、350、400、450 and 500 mg/L,norfloxacin 200、225、250、275 and 300 mg/L,enrofloxacin 25、50、75、100 and 125 mg/L.Under the observation of poisoning and death in 24,48 and 96 h,the guppies' safe concentrations of ciprofloxacin HCI,enrofloxacin and norfloxacin are: 300、200、25 mg/L,the minimum lethal oxygen concentration: 350、225、50 mg/L,half lethal concentration: 365、237、70 mg/L.The experiment result shows: enrofloxacin has the strongest toxicity,and then the norfloxacin,and ciprofloxacin HCl has the weakest.