The inhibition of condia of Botrytis cinerea by wuyiencin was observed on glass slides. The pathogenicity of the hyphae and conidia treated with wuyiencin and variation of enzymic activities associated with disease resistance in tomato sprayed with wuyiencin were also studied. The results showed that wuyiencin significantly inhibited the germination of the conidia of B. cinerea with EC50 as 14.1 μg/ml and completely inhibited by wuyiencin of 100 μg/ml. The pathogenicity of the hyphae and conidia of B. cinerea treated with wuyiencin (≥10 μg/ml) was significantly reduced. The increased activity of superoxide dismutase, peroxidase, polyphenol oxidase and phenylalanine ammoniam-lyase in tomato seedling upon treatment with wuyiencin of 100 μg/ml was detected. It is suggested that wuyiencin may trigger the defense reaction of tomato.