Study on the fingerprints of Honeysuckle and wild Honeysuckle by HPLC/UV/MS

Objective:To develop a method for studying on the HPLC/UV/MS fingerprints of Honeysuckle and Wild Honeysuckle and elucidating the major constituents contained in them. Methods: Separation was performed on Agilent ZORBAX SB C 18 (150mm×4.6mm,5μm) analytical column. The mobile phase consisted of 0.1% formic acid and methanol as gradient eluent. The flow rate was 1mL·min -1 . Results: Fingerprints obtained can be used for valuation of Honeysuckle and Wild Honeysuckle. 8 compounds contained in Honeysuckle and 5 compounds contained in Wild Honeysuckle were elucidated by HPLC/MS. Conclusion: The method can be used for the quality control of Honeysuckle and Wild Honeysuckle.