夏普
卵泡膜
凋亡抑制因子
卵泡闭锁
卵泡期
生物
卵巢
卵泡抑素
黄体
细胞凋亡
颗粒细胞
卵泡
男科
内分泌学
内科学
半胱氨酸蛋白酶
程序性细胞死亡
医学
生物化学
作者
Peng Han,Haiyun Xin,Jiahui Peng,Jinxing Hou,Lei Zhang,Yuxuan Song,Guang Li,Binyun Cao,Xiaopeng An
标识
DOI:10.1016/j.theriogenology.2017.04.048
摘要
X-linked inhibitor of apoptosis protein (XIAP), an endogenous of inhibitor of caspases, plays crucial roles in regulating ovarian granulosa cell apoptosis during follicular atresia. The aim of the present study was to determine the presence and localization of XIAP in the goat ovary and its expression level during follicular development. The full length cDNA of XIAP from goat ovary cells was cloned using reverse transcription PCR. A total of 497 amino acid residues were encoded by open reading frame and had high identity with homologous sequences from other mammals. XIAP was widely expressed in adult goat tissues as determined by real-time PCR and it demonstrated higher expression in propagative organs. High level of XIAP was detected in large healthy follicles and corpus luteum in comparison with that in small antral follicles, which was in accordance with the immunohistochemistry results and atretic follicles had very low expression. XIAP was localized in both granulosa and theca cells in antral follicles but not in primordial follicles. Furthermore, luteinizing hormone stimulated the proliferation of mRNA encoding XIAP in granulosa cells in vitro. The present study demonstrated that XIAP was expressed in a follicular-stage–dependent manner in goat ovaries.
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