In order to measure the low-frequency radiated sound power of the underwater sound source in a reverberation tank, the measuring method based on the characteristic acquisition of the testing sound field is proposed. Based on the normal-mode theory, the spatial averaging properties of the reverberation sound field with the ideal boundaries is firstly derived, of which the sound field transfer relationship from the reverberation field to the free field is obtained. In addition, for the purpose of obtaining the characteristic of the testing sound field with the general boundaries, the numerical method based on the ACTRAN software is utilized. According to obtaining the characteristic of the testing sound field in advance, the low-frequency radiated sound power can be got by measuring the square pressure in a reverberation tank. The proposed method is checked by the experiment measurement. The test results show that by utilizing the proposed method, the low-frequency radiated sound power of the underwater sound source has been accurately measured in the glass tank below the Schroeder cut-off frequency, of which the radiated sound power level deviation is less than 2 dB compared with the free field.