海马
生物能学
细胞生物学
糖酵解
生物
功能(生物学)
线粒体
调节器
焊剂(冶金)
化学
新陈代谢
生物化学
基因
动物
有机化学
作者
Zeinab Y. Motawe,Salma S. Abdelmaboud,Jerome W. Breslin
出处
期刊:Methods in molecular biology
日期:2023-10-01
卷期号:: 241-256
标识
DOI:10.1007/978-1-0716-3429-5_20
摘要
Endothelial bioenergetics have emerged as a key regulator of endothelial barrier function. Glycolytic parameters have been linked to barrier enhancement, and interruption with mitochondrial complexes was shown to disrupt endothelial barrier. Therefore, a new technology that has been introduced to assess bioenergetics and metabolism has also made it possible to determine roles of specific energy production pathways in endothelial health. The Seahorse extracellular flux analysis by Agilent technologies is a state of the art tool that has been more frequently used to evaluate bioenergetics of endothelial cells. This chapter includes details about different assays that can be used to study endothelial cells using the Seahorse analyzer and how interpretation of the results can provide novel insight about endothelial metabolism.
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