多路复用
微流控
背景(考古学)
纳米技术
癌细胞
材料科学
个性化医疗
癌症检测
精密医学
单细胞分析
表面增强拉曼光谱
光流学
拉曼散射
癌症
拉曼光谱
细胞
化学
生物信息学
生物
光学
物理
生物化学
遗传学
古生物学
作者
Kevin Oliveira,Alexandra Teixeira,José Moniz Fernandes,Cláudia Lorena Ribeiro Lopes,Alexandre Chícharo,Paulina Piairo,Lei Wu,Laura Rodríguez‐Lorenzo,Lorena Diéguez,Sara Abalde‐Cela
标识
DOI:10.1002/adom.202201500
摘要
Abstract In the context of personalized medicine, the analysis of single cells is key in order to understand the origin and evolution of cancer to provide an accurate prognosis. Microfluidics and microdroplets are increasingly used for the handling and understanding of the behavior of single cells, as they offer the perfect isolated environment. However, due to the small volumes handled, it is necessary to couple this technology with an ultrasensitive detection technique. Herein, surface‐enhanced Raman scattering (SERS) spectroscopy and droplet microfluidics are combined toward the multiplex phenotypic analysis of single cancer cells. For this, cancer cells are labeled with different SERS tags that recognize membrane proteins and encapsulated individually in microdroplets. Afterward, single cells within microdroplets are imaged by SERS spectroscopy. To the best of the authors’ knowledge, this is the first time that a multiplex phenotypic SERS analysis of single cells in microdroplets is shown. This integrated optofluidic platform paves the way toward the multiplex and automated characterization of cell populations in cancer patients.
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