离体
去细胞化
材料科学
生物医学工程
组织工程
再生医学
体内
纳米技术
干细胞
细胞生物学
生物
医学
生物技术
作者
Peizheng Wu,Hiroki Asada,Masataka Hakamada,Mamoru Mabuchi
标识
DOI:10.1002/adma.202209149
摘要
The development of tissue-like structures such as cell sheets, spheroids, and organoids has contributed to progress in regenerative medicine. Simultaneous achievement of scale up and high cell density of these tissues is challenging because sufficient oxygen cannot be supplied to the inside of large, high cell density tissues. Here, in vitro fabrication of vessels to supply oxygen to the inside of millimeter-sized scaffold-free tissues whose cell density is ≈200 million cells mL-1 , corresponding to those of native tissues, is shown. Hierarchical vascular networks by anastomosis of capillaries and a large vessel are essential for oxygen supply, whereas a large vessel or capillary networks alone make negligible contributions to the supply. The hierarchical vascular networks are formed by a top-down approach, which offers a new option for ex vivo whole organs without decellularization and 3D-bioprinting.
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