CpG站点
CpG寡核苷酸
Treg细胞
RAR相关孤儿受体γ
FOXP3型
化学
免疫系统
生物
免疫学
白细胞介素2受体
分子生物学
细胞生物学
流式细胞术
癌症研究
TLR9型
关贸总协定3
细胞因子
作者
Hailing Liu,Yuqiang Ji,Xiao-Rong Ma,Aili He,Wan-Hong Zhao,Pengyu Zhang,Liufang Gu,Bo Lei,Yi-Lin Zhang,Yueli Wang,Wanggang Zhang,Jin Wang
标识
DOI:10.1016/j.molimm.2021.01.003
摘要
Abstract Aim The balance between Th17 cells and T regulatory (Treg) cells has emerged as a prominent factor in regulating cancer development. However, the effect of CpG oligodeoxynucleotides (ODNs) on the differentiation of Treg/Th17 cells has not been well studied. We sought here to explore the function of CpG ODNs in the differentiation of Tregs and Th17 cells in vitro and in vivo. Methods Mouse spleen cells were cultured with anti-CD3 monoclonal antibodies in vitro. Tregs and Th17 cell differentiation was induced by transforming growth factor (TGF)-β and interleukin (IL)-2, or TGF-β, IL-6, and IL-23, respectively. Then cells were treated with two CpG ODNs, CpG 1982, or CpG 1826. FBL-3-inoculated C57Bl/6 mice were treated with CpG 1826, tumor vaccine, or combination of CpG 1826 and tumor vaccine. After treatment, spleen cells and serum were isolated, and Tregs/Th17 cells were detected by flow cytometry. The expression of forkhead box P3 (Foxp3), retinoid-related orphan receptor gamma-t (RORγt), IL-10, and IL-17 mRNA was measured by real-time PCR, and protein levels were measured by Western blot and enzyme-linked immunosorbent assay. Results The frequency of Treg cells increased significantly (p Conclusion CpG 1826 may inhibit the differentiation of Treg cells induced by cytokines, promote the differentiation of Th17 cells in vitro and in murine leukemia models, and prolong the median survival of mice with leukemia.
科研通智能强力驱动
Strongly Powered by AbleSci AI