Techniques and Applications in Nanopore and Long Read Sequencing

Since the introduction of nanopore sequencing in 2013, countless new methods and techniques have been developed that has enabled researcher to study nucleic acids like never before. Nanopore sequencing has many characteristics including ultra-long DNA sequencing of single read lengths of up to a megabase, direct RNA sequencing with modified base detection, and amplicon sequencing, to name a few. These sequencing systems come in many formats for both small and large core laboratories as well a multitude of library synthesis methods and barcoding options to address many techniques required for many aspects of both genomic and transcriptional studies. In this session we will discuss some of the popular techniques as well as an open panel discussion for audience interaction. Speaker 1: Scott Tighe : Introduction to Nanopore Sequencing 5 min Speaker 2: High Molecular Weight DNA Extractions for Long Read Sequencing and Other Genomic Applications Kelvin Liu Circulomics 20 min Speaker 3: Detection of Base Modifications using Nanopore Sequencing Jonathan Foox Weill Cornell 20 Min Speaker 4: Efficient de novo assembly of eleven human genomes using PromethION sequencing and a novel nanopore toolkit Miten Jain Univ of Cali Santa Cruz 20 Min Part 5: Open Panel Discussion: 20 Min.