二聚体
免疫球蛋白Fab片段
化学
结晶学
晶体结构
分子
立体化学
二价(发动机)
分子置换
作者
Salman Shahid,Mingming Gao,D. Travis Gallagher,Edwin Pozharski,Robert G. Brinson,Zhen-Yong Keck,Steven K. H. Foung,Thomas R. Fuerst,Roy A. Mariuzza
标识
DOI:10.1016/j.jmb.2020.11.013
摘要
• The Fab of human anti-HCV antibody HC84.26.5D forms bivalent dimers in solution. • Crystal structure revealed that Fab HC84.26.5D is a domain-swapped dimer. • Dimerization is mediated by deletion of one residue in the H chain elbow region. • Domain-swapped Fab dimers are useful as fiducial markers for cryoEM. We determined the crystal structure to 1.8 Å resolution of the Fab fragment of an affinity-matured human monoclonal antibody (HC84.26.5D) that recognizes the E2 envelope glycoprotein of hepatitis C virus (HCV). Unlike conventional Fabs, which are monovalent monomers, Fab HC84.26.5D assembles into a bivalent domain-swapped dimer in which the two V L /V H modules are separated by ~25 Å. In solution, Fab HC84.26.5D exists predominantly as a dimer (~80%) in equilibrium with the monomeric form of the Fab (~20%). Dimerization is mediated entirely by deletion of a single residue, V H Ser113 (Kabat numbering), in the elbow region linking the V H and C H 1 domains. In agreement with the crystal structure, dimeric Fab HC84.26.5D is able to bind two HCV E2 molecules in solution. This is only the second example of a domain-swapped Fab dimer from among >3000 Fab crystal structures determined to date. Moreover, the architecture of the doughnut-shaped Fab HC84.26.5D dimer is completely different from that of the previously reported Fab 2G12 dimer. We demonstrate that the highly identifiable shape of dimeric Fab HC84.26.5D makes it useful as a fiducial marker for single-particle cryoEM analysis of HCV E2. Bivalent domain-swapped Fab dimers engineered on the basis of HC84.26.5D may also serve as a means of doubling the effective size of conventional Fab–protein complexes for cryoEM.
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