Optimization of Chickpea Protein Extraction Using Response Surface Methodology

The purpose of the present study was to optimize alkali protein extraction from defatted chickpea using one-factor-at-a-a-time method and response surface methodology.The results showed that all investigated parameters had a notable effect on the extraction yield of chicken protein and could be ranked in decreasing order of importance in their effects as follows: pH liquid-to-material ratioextraction time.The optimal extraction parameters were pH 11.0 and 17.7:1(mL/g) of liquid-to-material ratio for 88.4 min of extraction at 20 ℃,resulting in an extraction yield of 82.33%.The established regression model describing the extraction yield of chicken protein as a function of three extraction parameters was highly significant(R2=0.9630).The predictive and experimental results were found to be in good agreement.Thus,the model was applicable for the prediction of protein yield.