Effect of the Exogenous Cellulase on the Activity of its Native Enzyme in Soil under Same Temperature Condition

[Objective] The activity of the native cellulase in soil and the variation of its enzymatic hydrolysis to the substrate was measured after the exogenous cellulase was added in the soil.[Method] The maize being taken as the reaction substrate,the effect of the exogenous cellulase added in soil on the activity of native enzyme and its enzymatic hydrolysis to the substrate was explored under the environmental condition of the pH value of 6.5,the temperature of 27 ℃,37 ℃ or 47 ℃.[Results] Under the environmental condition of the pH value of 6.5,the activity of enzyme was firstly raised and then decreased,which was possible that the speed of enzymatic hydrolysis could be fasted under the coexist of soil and enzyme,thus,most of straws had been hydrolyzed in early period and in the later,few remains of straws resulted in the reduction of the speed of enzymatic hydrolysis.The order of the activity of enzyme under the temperature was 47 ℃37 ℃27 ℃ and under the treatment of temperature,the activity of native enzyme in soil could be promoted due to the addition of the exogenous cellulase.[Conclusion] The addition of the exogenous cellulose was beneficial to the improvement of the activity of native enzyme in soil.