Study on the Expression of Bt Insecticidal Protein of the Transgenic Insect-resistant Corn

An enzyme-linked immunosorbent assay(ELISA) method was used to detect and quantitate the expression of Bacillus thuringiensis(Bt) insecticidal protein in different growth periods and different tissues of the Bt transgenic insect-resistant corn.From the results,we can see that the expressions of Bacillus thuringiensis(Bt) insecticidal protein in leaves are the highest,while the lowest in pollen.With the growth of the plant,the expression of Bacillus thuringiensis(Bt) insecticidal protein in leaves,stalk and ear continuously increased.The smallest concentration of Bt protein for detection is 0.5 ng/mL.