Study on Liquid Fermentation Conditions of Monascus Pigment by Monascus fumeus

[Objective] To determine the optimal culture medium and fermentation conditions of Monascus pigment production by Monascus fumeus.[Method]By changing single variable,the variation law of red pigment was determined,the optimal culture medium formula was determined.[Result]The optimal culture medium was as follows: glucose 5%,yeast powder 2%,CaCO30.2%,NaCl 0.1%,MgSO4·7H2O0.1%,KH2PO40.2%,Tween 20 0.05%.The suitable fermentation conditions were as follows: pH 6.0,broth volume of the shake flake 60ml /250 ml,inoculation volume 10%( 1 /6),seed-age 72 h,culture temperature 30 ℃,rotation speed 180 r /min,fermentation time 6 d,the Monascus pigment reached 19.2 U /ml.[Conclusion]The study can provide theoretical basis for the large scale production of Monascus pigment.