Objective: A method was established to determine the content of phenylethanoid glycosides in total Cistanchis glycosides capsule. Methods: UV spectrophotometry was used for determination,λ max =333 nm. Result: The average content of phenylethanoid glycosides was 20.45 mg/grain, the recovery was 99.36% and the RSD was 1.17%. Conclusion: The method is simple and sensitive.