Study of Metabolic Conversion of Mogrol Glycosides in Fruit of Siraitia Grosvenorii

Objective: Studying metabolic conversion of mogrol glycosides in the tissue culture related Siraitia grosvenorii fruit during the course of fruit maturity,so that key clues could be found for the research of mogrol glycosides associated metabolic enzymes.Method:Mogrol glycosides were extracted from Siraitia grosvenorii fruit of different growing ages by boiling water with microwave oven.Mogrol glycosidesⅡ,Ⅲ,Ⅴ were determined by HPLC.Result: Bitter mogrol glycoside Ⅱ and tasteless mogrol glycoside Ⅲ were 3 666.4μg/100mg and 48.4μg/100mg respectively,without mogrol glycosideⅤ detected in 10 day-old fruit.Magrol glycoside Ⅲ was 1 213.8μg/100mg and mogrol glycosideⅤ was detected 95.1μg/100mg in 50 day-old fruit. Mogrol glycosideⅤ increased rapidly and reached high level.1 331.4μg/100mg without mogrol glycosidesⅡand Ⅲ detected in 70dayold fruit.Conclusion: High glucose sweet mogrol glycosides might come from low glucose bitter mogrol glycosides via metabolic conversion.This conclusion can provide scientific evidence for the study of glycosyl transferase of Siraitia grosvenorii mogrol glycosides.