Objective:In order to acquire the information of mycoplasma infection on urogenital tract and in vitro drug susceptibility.Method:The isolation?cultivation?identification and susceptivity for drugs of mycoplasma,all of these items were examined at once with the kit of mycoplasma.Results:We found 112 cases positive mycoplasma from 268 patients with urogenital tract infection,the positive rate is 41.79%.In these studies we found two strains(1.79%)of mycoplasma that resisted to 10 kinds of antimicrobial agents.Conclusion:The rate of mycoplasma infection on urogenital tract is 41.79%,the highest is ureaplasma urealyticum.The antibiotic effect of macrolides on mycoplasma is better than that of chloranphenicol and tetracycoines,and that of quinolones is poor.