羟基烷酸
生物塑料
结晶度
热重分析
傅里叶变换红外光谱
嗜盐菌
材料科学
核化学
差示扫描量热法
分析化学(期刊)
化学
色谱法
化学工程
结晶学
有机化学
生物
细菌
物理
遗传学
热力学
工程类
生态学
作者
Riddhi Mahansaria,Anusua Dhara,Amit Saha,Sumanta Haldar,Joydeep Mukherjee
标识
DOI:10.1016/j.ijbiomac.2017.10.009
摘要
Application of halophiles can decrease the cost of polyhydroxyalkanoate (PHA) production or bioplastic which are an alternative to the petroleum-derived plastic. Extremely halophilic archaeon, Natrinema ajinwuensis RM-G10 accumulated 61.02 ± 0.68% PHA of its cell dry mass at 72 h in repeated batch cultures yielding 0.210 ± 0.001 g L−1 h−1 volumetric productivity after selection of the best cultivation conditions. Transmission electron microscopy showed the presence of PHA granules inside the archaeal cells. Characterization by gas chromatographic analysis, gas chromatographic- mass spectrophotometric analysis, thermogravimetric analysis, differential scanning calorimetric analysis, X-ray diffraction analysis, Fourier transform infra red spectroscopy and nuclear magnetic resonance spectroscopy revealed the polymer to be poly(3-hydroxybutyrate-co-3-hydroxyvalerate) with 13.93 mol% 3-hydroxyvalerate content and having 35.45% crystallinity, −12.3 °C glass transition temperature, 143 °C and 157.5 °C melting temperatures and 284 °C degradation temperature. This is the first report on production enhancement (on a small scale) and characterization of the polyhydroxyalkanoate produced by Natrinema ajinwuensis (as synonym) ≡ Natrinema altunense strain RM-G10 and the Natrinema genus in general.
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