检出限
电化学
化学
唾液
尿
左氧氟沙星
电极
色谱法
循环伏安法
生物化学
物理化学
抗生素
作者
Hamid Reza Lotfi Zadeh Zhad,Rebecca Y. Lai
标识
DOI:10.1002/elan.201700428
摘要
Abstract We report the design of an electrochemical sensor capable of detecting levofloxacin (LEVX) in complex biological samples. This detection strategy is simple, fast, and does not require sample pretreatment or electrode modification. Unlike previously developed electrochemical LEVX sensors that require direct oxidation of LEVX, the sensing mechanism is based on the complexation reactions between LEVX and iron(III), resulting in a concentration‐dependent decrease in the iron(III) reduction peak current and a shift in the peak potential. These changes are presumably attributed to the decrease in the concentration of uncomplexed Fe(III) in the solution. The concentration‐dependent change in both the current and potential can be used for quantification of LEVX in various samples, including 50 % synthetic urine and 25 % synthetic human saliva. The limit of detection was estimated to be in the range of 1.5 to 2.3 μM, concentrations that are much lower than the concentration of LEVX found in urine and saliva samples of patients administered this drug for conditions such as urinary tract infection. With further optimization, this sensing strategy could find applications in clinical pharmacokinetic studies.
科研通智能强力驱动
Strongly Powered by AbleSci AI