Epstein-Barr Virus as a Potential Etiology of Persistent Bladder Inflammation in Human Interstitial Cystitis/Bladder Pain Syndrome

No AccessJournal of UrologyAdult Urology1 Sep 2018Epstein-Barr Virus as a Potential Etiology of Persistent Bladder Inflammation in Human Interstitial Cystitis/Bladder Pain Syndrome Jia-Fong Jhang, Yung-Hsiang Hsu, Chih-Wen Peng, Yuan-Hong Jiang, Han-Chen Ho, and Hann-Chorng Kuo Jia-Fong JhangJia-Fong Jhang Department of Urology, Buddhist Tzu Chi General Hospital and Tzu Chi University, Hualien, Taiwan , Yung-Hsiang HsuYung-Hsiang Hsu Department of Pathology, Buddhist Tzu Chi General Hospital and Tzu Chi University, Hualien, Taiwan , Chih-Wen PengChih-Wen Peng Institute of Medical Sciences and Department of Life Sciences, Tzu Chi University, Hualien, Taiwan , Yuan-Hong JiangYuan-Hong Jiang Department of Urology, Buddhist Tzu Chi General Hospital and Tzu Chi University, Hualien, Taiwan , Han-Chen HoHan-Chen Ho Department of Anatomy, Tzu Chi University, Hualien, Taiwan , and Hann-Chorng KuoHann-Chorng Kuo Department of Urology, Buddhist Tzu Chi General Hospital and Tzu Chi University, Hualien, Taiwan View All Author Informationhttps://doi.org/10.1016/j.juro.2018.03.133AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: Interstitial cystitis/bladder pain syndrome is characterized by bladder inflammation without bacterial infection. Although viral infection is a potential etiological cause, few studies have been reported. Materials and Methods: Bladder specimens were obtained from patients with interstitial cystitis/bladder pain syndrome and from patients with stress urinary incontinence as controls. Bladder specimens were tested for Epstein-Barr encoded RNAs by in situ hybridization and for Epstein-Barr DNA by quantitative real-time polymerase chain reaction, serology and immunohistochemical staining. Results: Enrolled in study were 16 patients with interstitial cystitis/bladder pain syndrome and Hunner lesions, 23 without interstitial cystitis/bladder pain syndrome or Hunner lesions and 10 controls. The positive rate of Epstein-Barr encoded RNA on in situ hybridization in bladder specimens from patients with vs without interstitial cystitis/bladder pain syndrome and Hunner lesions was 50% vs 8.6%. No Epstein-Barr encoded RNA was found in control specimens. On quantitative real-time polymerase chain reaction Epstein-Barr DNA was detected in 68.8% vs 16.7% of bladder specimens in patients with vs without interstitial cystitis/bladder pain syndrome and Hunner lesions. The median viral load was 1,836 copies per ml (range 216 to 75,144). Only 1 control specimen was Epstein-Barr positive on quantitative real-time polymerase chain reaction. All serum samples from patients with interstitial cystitis/bladder pain syndrome showed past Epstein-Barr viral infection. Epstein-Barr infection was present in 87.5% vs 17.4% of bladder specimens from patients with vs without interstitial cystitis/bladder pain syndrome and Hunner lesions for a total of 46.2% with interstitial cystitis/bladder pain syndrome. Immunohistochemical staining of CD3 and CD20 revealed that Epstein-Barr infection was mainly restricted to T lymphocytes in bladders showing interstitial cystitis/bladder pain syndrome. 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Link, Google Scholar © 2018 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetailsCited byJhang J, Hsu Y, Jiang Y, Ho H and Kuo H (2020) Clinical Relevance of Bladder Histopathological Findings and Their Impact on Treatment Outcomes among Patients with Interstitial Cystitis/Bladder Pain Syndrome: An Investigation of the European Society for the Study of Interstitial Cystitis Histopathological ClassificationJournal of Urology, VOL. 205, NO. 1, (226-235), Online publication date: 1-Jan-2021.Lai H, Pickersgill N and Vetter J (2020) Hunner Lesion Phenotype in Interstitial Cystitis/Bladder Pain Syndrome: A Systematic Review and Meta-AnalysisJournal of Urology, VOL. 204, NO. 3, (518-523), Online publication date: 1-Sep-2020.Akiyama Y, Maeda D, Katoh H, Morikawa T, Niimi A, Nomiya A, Sato Y, Kawai T, Goto A, Fujimura T, Fukuhara H, Nakagawa T, Igawa Y, Ishikawa S, Fukayama M, Kume H and Homma Y (2019) Molecular Taxonomy of Interstitial Cystitis/Bladder Pain Syndrome Based on Whole Transcriptome Profiling by Next-Generation RNA Sequencing of Bladder Mucosal BiopsiesJournal of Urology, VOL. 202, NO. 2, (290-300), Online publication date: 1-Aug-2019.Smith J (2018) This Month in Adult UrologyJournal of Urology, VOL. 200, NO. 3, (468-469), Online publication date: 1-Sep-2018. Volume 200Issue 3September 2018Page: 590-596Supplementary Materials Advertisement Copyright & Permissions© 2018 by American Urological Association Education and Research, Inc.Keywordsurinary bladderinterstitialetiologyT-lymphocytesEpstein-Barr virus infectionscystitisMetricsAuthor Information Jia-Fong Jhang Department of Urology, Buddhist Tzu Chi General Hospital and Tzu Chi University, Hualien, Taiwan Equal study contribution. More articles by this author Yung-Hsiang Hsu Department of Pathology, Buddhist Tzu Chi General Hospital and Tzu Chi University, Hualien, Taiwan Equal study contribution. More articles by this author Chih-Wen Peng Institute of Medical Sciences and Department of Life Sciences, Tzu Chi University, Hualien, Taiwan More articles by this author Yuan-Hong Jiang Department of Urology, Buddhist Tzu Chi General Hospital and Tzu Chi University, Hualien, Taiwan More articles by this author Han-Chen Ho Department of Anatomy, Tzu Chi University, Hualien, Taiwan More articles by this author Hann-Chorng Kuo Department of Urology, Buddhist Tzu Chi General Hospital and Tzu Chi University, Hualien, Taiwan More articles by this author Expand All Advertisement PDF downloadLoading ...