二硫化钼
检出限
生物传感器
电化学
插层(化学)
图层(电子)
剥离(纤维)
线性范围
纳米技术
亚甲蓝
DNA
材料科学
色谱法
化学
组合化学
无机化学
电极
生物化学
物理化学
冶金
催化作用
复合材料
光催化
作者
Dujuan Li,Fei Wen,Gaofeng Wang
出处
期刊:Analytical Methods
[The Royal Society of Chemistry]
日期:2022-01-01
卷期号:14 (20): 1956-1962
被引量:4
摘要
An electrochemical biosensor based on few-layer molybdenum disulfide (MoS2) nanosheets was fabricated for the highly sensitive detection of tumor marker circulating tumor DNA (ctDNA) in this paper. The MoS2 nanosheets with few layers were prepared by the shear stripping. Compared with the mechanical stripping method and the lithium ion intercalation method, this method is simpler to operate, and the prepared MoS2 nanosheets had good electrochemical activity. The biosensing platform was fabricated based on the discriminative affinity of MoS2 nanosheets towards single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA). Methylene blue (MB) was used as the signal molecule. The results showed that the detection of ctDNA by this sensor showed an excellent linear relationship in the concentration range of 1.0 × 10-7 M to 1.0 × 10-16 M, and the detection limit was 2.5 × 10-18 M. In addition, this sensor exhibited outstanding stability and specificity. This strategy provides an alternative approach for ctDNA detection and an effective sensing strategy for future in vitro cancer diagnosis by label-free detection.
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