Erteng Tongbi Decoction ameliorates collagen-induced arthritis in mice via modulating T cell differentiation and cytokines balance

传统医学 关节炎 汤剂 医学 炎症 口服 促炎细胞因子 类风湿性关节炎 药理学 免疫学 脾脏
作者
Mengjiali Wei,SU Jia-yan,Qian Ma,Fang Zhang,Jing Wang,Liang Feng,Xiao‐Bin Jia,Xiaobin Tan
出处
期刊:Journal of Ethnopharmacology [Elsevier]
卷期号:286: 114928-114928 被引量:9
标识
DOI:10.1016/j.jep.2021.114928
摘要

Herbs have been commonly used for the treatment of rheumatoid arthritis (RA). It has been verified that Erteng Tongbi Decoction has good therapeutic effects on RA, while, relatively few studies on the relationship between its components and anti-rheumatoid efficacy were carried out.To discuss the anti-RA effects of Erteng Tongbi Decoction on collagen-induced arthritis (CIA) in mice and the influence of T cell differentiation and cytokines balance.Separate researches on the two traditional Chinese medicines of the Erteng Tongbi Decoction were conducted. First, a murine peritoneal macrophage model was established, and then the cytokines levels and macrophage maturity were measured to select the best extraction solvent. Furthermore, ethanol extracts were partitioned successively with four kinds of solvents, and the anti-inflammatory parts were selected by the same vitro model. Subsequently, mice were arbitrarily divided into control, CIA model, positive control, effective parts alone or in combination. After 20 days of oral administration, the weight, hind paw volume, rheumatism index value, and the pathological changes were checked to assess the obvious level of arthritis. Furthermore, the levels of IL-6, TNF-α, IL-10, and IL-17A in serum and the balance of Th17/Treg and Th1/Th2 cells in spleen and mesenteric lymph nodes (MLN) was detected. Finally, the major active constituents were identified.In vitro, the anti-inflammatory effects of ethanol extracts was much better than water extract. In addition, the effective parts of Celastrus orbiculatus Thunb. ethanol extract were petroleum ether parts and dichloromethane parts. The effective parts of Spatholobus suberectus Dunn. ethanol extracts was petroleum ether parts and ethyl acetate parts screened. In vivo, effective parts compatibility could inhibit the progression of inflammation by modulating T cell differentiation and cytokines balance. Constituent analysis revealed that effective parts contained sesquiterpenes alkaloids, phenolic acids, and flavanols.Erteng Tongbi Decoction could notably ameliorate CIA mice by modulating T cell differentiation and cytokines balance and support its application in folk medicine.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
hhh2018687完成签到,获得积分10
2秒前
天才罗完成签到 ,获得积分10
3秒前
俊逸吐司完成签到 ,获得积分10
5秒前
萨格完成签到 ,获得积分10
10秒前
meiyang完成签到 ,获得积分10
13秒前
kento应助蝌蚪采纳,获得200
13秒前
科研通AI2S应助蝌蚪采纳,获得10
20秒前
哭泣的映寒完成签到 ,获得积分10
30秒前
mrwang完成签到 ,获得积分10
34秒前
mia完成签到,获得积分10
38秒前
41秒前
稳重的秋天完成签到,获得积分20
43秒前
YC发布了新的文献求助10
47秒前
乃惜完成签到,获得积分10
58秒前
YC完成签到,获得积分10
1分钟前
细心的如天完成签到 ,获得积分10
1分钟前
Aurora完成签到 ,获得积分10
1分钟前
btcat完成签到,获得积分10
1分钟前
丹青完成签到 ,获得积分10
1分钟前
pphss完成签到,获得积分10
1分钟前
Jack80发布了新的文献求助200
1分钟前
zgtmark完成签到,获得积分10
1分钟前
温暖完成签到 ,获得积分10
1分钟前
碧蓝雁风完成签到 ,获得积分10
1分钟前
Kidmuse完成签到,获得积分10
1分钟前
困困困完成签到 ,获得积分10
1分钟前
ylkk完成签到 ,获得积分10
1分钟前
nuliguan完成签到 ,获得积分10
1分钟前
1分钟前
晚街听风完成签到 ,获得积分10
2分钟前
franca2005完成签到 ,获得积分10
2分钟前
kxdxng完成签到,获得积分10
2分钟前
婳祎完成签到 ,获得积分10
2分钟前
喵了个咪完成签到 ,获得积分10
2分钟前
烽烽烽发布了新的文献求助30
2分钟前
skippy完成签到 ,获得积分10
2分钟前
yy完成签到 ,获得积分10
2分钟前
t铁核桃1985完成签到 ,获得积分10
2分钟前
dhdhg完成签到 ,获得积分10
2分钟前
wguanmc完成签到,获得积分10
2分钟前
高分求助中
좌파는 어떻게 좌파가 됐나:한국 급진노동운동의 형성과 궤적 2500
Sustainability in Tides Chemistry 1500
TM 5-855-1(Fundamentals of protective design for conventional weapons) 1000
Cognitive linguistics critical concepts in linguistics 800
Threaded Harmony: A Sustainable Approach to Fashion 799
Livre et militantisme : La Cité éditeur 1958-1967 500
氟盐冷却高温堆非能动余热排出性能及安全分析研究 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3052652
求助须知:如何正确求助?哪些是违规求助? 2709874
关于积分的说明 7418267
捐赠科研通 2354453
什么是DOI,文献DOI怎么找? 1246090
科研通“疑难数据库(出版商)”最低求助积分说明 605951
版权声明 595921