How does the polyhexamethylene guanidine interact with waste activated sludge and affect the metabolic functions in anaerobic fermentation for volatile fatty acids production

Antibacterial agents are frequently used to ensure public hygiene. Most of the massively consumed chemicals are discarded and accumulated in waste activated sludge (WAS), which might influence the subsequent anaerobic fermentation process for WAS treatment. This study mainly investigated the impacts of polyhexamethylene guanidine (PHMG, considered as a safe and efficient broad-spectrum antibacterial agent) on the volatile fatty acids (VFAs) production derived from WAS anaerobic fermentation and disclosed the key mechanisms. Results demonstrated that low level of PHMG evidently increased the VFAs accumulation as well as the acetic acid proportion, while the excessive dose posed evident negative effects. Further analysis found that appropriate PHMG synchronously stimulated the solubilization/hydrolysis and acidification processes but inhibited methanogenesis. Mechanistic exploration revealed that PHMG firstly absorbed on WAS due to electric attraction but then interacted with WAS to promote extracellular polymeric substance (EPS) disintegration and organics release (especially proteinaceous matter). Moreover, PHMG affected the microbial community structure and metabolic functions. The low level of PHMG evidently enriched functional VFAs producers ( i.e., Desulfobulbus, Macellibacteroides and Sporanaerobacter ) and upregulated the critical genes expression responsible for substrates metabolism (particularly the proteins) and VFAs biosynthesis ( i.e., aldehyde dehydrogenase (NAD+) (K00128) and molybdopterin oxidoreductase (K00184)). This study provides an in-depth understanding of emerging pollutant impacts on WAS fermentation and provides insightful guidance on WAS disposal. • PHMG exhibits dose-dependent effects on the VFAs production during WAS fermentation. • PHMG enhances solubilization/hydrolysis and acidification stages but inhibit methanation. • The interactions of PHMG and WAS are disclosed. • PHMG contributes to the EPS destruction and organics (mainly protein) release. • The functional anaerobes and metabolic traits related to VFAs are improved.