乙型肝炎病毒
肝细胞癌
病毒学
病毒
无症状携带者
基因
生物
慢性感染
抗原
医学
免疫学
癌症研究
疾病
免疫系统
遗传学
内科学
作者
Fan Shen,Ih Jen Su,Han Chieh Wu,Yi Hsuan Hsieh,Wei Jen Yao,Kung Chia Young,Tsung Chuan Chang,Hui Chuan Hsieh,Han Ni Tsai,Wenya Huang
标识
DOI:10.1186/1423-0127-16-84
摘要
Chronic hepatitis B virus (HBV) infection is an important cause of hepatocellular carcinoma (HCC) worldwide. The pre-S1 and -S2 mutant large HBV surface antigen (LHBS), in which the pre-S1 and -S2 regions of the LHBS gene are partially deleted, are highly associated with HBV-related HCC.The pre-S region of the LHBS gene in two hundred and one HBV-positive serum samples was PCR-amplified and sequenced. A pre-S oligonucleotide gene chip was developed to efficiently detect pre-S deletions in chronic HBV carriers. Twenty serum samples from chronic HBV carriers were analyzed using the chip.The pre-S deletion rates were relatively low (7%) in the sera of patients with acute HBV infection. They gradually increased in periods of persistent HBV infection: pre-S mutation rates were 37% in chronic HBV carriers, and as high as 60% in HCC patients. The Pre-S Gene Chip offers a highly sensitive and specific method for pre-S deletion detection and is less expensive and more efficient (turnaround time 3 days) than DNA sequencing analysis.The pre-S1/2 mutants may emerge during the long-term persistence of the HBV genome in carriers and facilitate HCC development. Combined detection of pre-S mutations, other markers of HBV replication, and viral titers, offers a reliable predictive method for HCC risks in chronic HBV carriers.
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