癌细胞
细胞生长
化学
胱氨酸
癌症
癌症研究
雌激素受体
谷胱甘肽
半胱氨酸
细胞生物学
生物化学
生物
乳腺癌
内科学
医学
酶
作者
Yuzhe Yang,Douglas Yee
标识
DOI:10.1158/0008-5472.can-13-1803
摘要
Abstract Insulin-like growth factors (IGF) stimulate cell growth in part by increasing amino acid uptake. xCT (SLC7A11) encodes the functional subunit of the cell surface transport system xC−, which mediates cystine uptake, a pivotal step in glutathione synthesis and cellular redox control. In this study, we show that IGF-I regulates cystine uptake and cellular redox status by activating the expression and function of xCT in estrogen receptor-positive (ER+) breast cancer cells by a mechanism that relies on the IGF receptor substrate-1 (IRS-1). Breast cancer cell proliferation mediated by IGF-I was suppressed by attenuating xCT expression or blocking xCT activity with the pharmacologic inhibitor sulfasalazine (SASP). Notably, SASP sensitized breast cancer cells to inhibitors of the type I IGF receptor (IGF-IR) in a manner reversed by the reactive oxygen species (ROS) scavenger N-acetyl-L-cysteine. Thus, IGF-I promoted the proliferation of ER+ breast cancer cells by regulating xC− transporter function to protect cancer cells from ROS in an IRS-1–dependent manner. Our findings suggest that inhibiting xC− transporter function may synergize with modalities that target the IGF-IR to heighten their therapeutic effects. Cancer Res; 74(8); 2295–305. ©2014 AACR.
科研通智能强力驱动
Strongly Powered by AbleSci AI