精氨酸酶
精氨酸
一氧化氮
一氧化氮合酶
内分泌学
内科学
脾脏
肿瘤坏死因子α
细胞因子
化学
医学
生物化学
氨基酸
作者
Iara Eliza Pacífico Quirino,Mariângela Carneiro,Valbert Nascimento Cardoso,Rosana das Graças Carvalho dos Santos,Leda Quércia Vieira,Jacqueline Araújo Fiúza,Jacqueline I. Alvarez-Leite,Simone de Vasconcelos Generoso,María Isabel Toulson Davisson Correia
标识
DOI:10.1177/0148607114546374
摘要
The purpose of this study was to assess the effect of arginine supplementation on arginase activity, tumor necrosis factor-α (TNF-α) and interleukin-10 (IL-10) synthesis in cultured splenic macrophages from a murine model of intestinal obstruction (IO). The effects of nitric oxide synthase (iNOS) inhibition were also studied using iNOS knockout animals.Male C57BL6/J wild-type (WT) and iNOS knockout (iNOS-/-) mice were randomized into 6 groups: Sham and Sham-/- (standard chow), IO and IO-/- (standard chow + IO), and Arg and Arg-/- (standard chow supplemented with arginine + IO). After 7 days of treatment with standard or supplemented chow, IO was induced. Arginase activity as well as TNF-α and IL-10 levels were analyzed in splenic macrophage cultures.Arginine supplementation and the absence of iNOS increased arginase activity in splenic macrophages (Arg, IO-/-, and Arg-/- groups vs the Sham group; P < .05). Arginine was also related to a decrease in TNF-α levels (Arg vs IO group, P < .05) and maintenance of IL-10 levels (Arg vs other groups, P > .05). The inhibition of iNOS did not result in effects on the concentration of cytokines (Sham-/-, IO-/-, and Arg-/- vs other, P < .05).Arginine supplementation and iNOS inhibition led to increased arginase activity. Arginine availability decreased plasma TNF-α levels, which may be directly related to nitric oxide derived from arginine.
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