T lymphoma variants with specifically altered growth in semi‐solid media

Abstract The relationship between the tumorigenic potential and the cloning efficiency of T lymphoma BW5147 in semi‐solid media has been studied. Two stable variants exhibiting a 30‐fold decrease of their cloning efficiencies in agarose an methycellulose media were independently isolated by negative selection with FuDr. These variants show no alteration of their growth properties in liquid medium and are still able to proliferate in liquid suspension over a bottom layer of agarose. This new phenotype is not correlated with any decreased tumorigenicity in syngeneic AKR/J mice.