化学
赖氨酸
组合化学
荧光
噬菌体
区域选择性
共轭体系
核酸
氨基酸
谷氨酸
分子
表面改性
天冬氨酸
生物化学
有机化学
聚合物
大肠杆菌
催化作用
物理化学
物理
基因
量子力学
作者
Kai Li,Yi Chen,Siqi Li,Huong Giang Nguyen,Zhongwei Niu,Sylvaine You,Charlene M. Mello,Xiao‐Bing Lu,Qin Wang
摘要
The M13 bacteriophage has been demonstrated to be a robust scaffold for bionanomaterial development. In this paper, we report on the chemical modifications of three kinds of reactive groups, i.e., the amino groups of lysine residues or N-terminal, the carboxylic acid groups of aspartic acid or glutamic acid residues, and the phenol group of tyrosine residues, on M13 surface. The reactivity of each group was identified through conjugation with small fluorescent molecules. Furthermore, the regioselectivity of each reaction was investigated by HPLC-MS-MS. By optimizing the reaction condition, hundreds of fluorescent moieties could be attached to create a highly fluorescent M13 bacteriophage. In addition, cancer cell targeting motifs such as folic acid could also be conjugated onto the M13 surface. Therefore, dual-modified M13 particles with folic acid and fluorescent molecules were synthesized via the selective modification of two kinds of reactive groups. Such dual-modified M13 particles showed very good binding affinity to human KB cancer cells, which demonstrated the potential applications of M13 bacteriophage in bioimaging and drug delivery.
科研通智能强力驱动
Strongly Powered by AbleSci AI