调节器
生物
大肠杆菌
次黄嘌呤
嘌呤
鸟嘌呤
鉴定(生物学)
基因
抑制因子
嘌呤代谢
遗传学
生物化学
微生物学
转录因子
核苷酸
植物
酶
作者
Li Mei Meng,Per Nygaard
标识
DOI:10.1111/j.1365-2958.1990.tb00580.x
摘要
Summary Addition of purine compounds to the growth medium of Escherichia coli and Salmonella typhimurium causes repressed synthesis of the purine biosynthetic enzymes. The repression is mediated through a regulatory protein, PurR. To identify the co‐repressor(s) of PurR, two approaches were used: (i) mutations were introduced into purine salvage genes and the effects of different purines on pur gene expression were determined; (ii) purine compounds which dictate the binding of the PurR protein to its operator DNA were resolved by gel retardation. Both the in vivo and the in vitro data indicated that guanine and hypoxanthine are co‐repressors. The toxic purine analogues 6‐mercaptopurjne and 6‐thioguanine also activated the binding of PurR to its operator DNA.
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