化学
过氧化氢
乙醇氧化酶
甲醇
试剂
连锁反应
酒
光化学
组合化学
有机化学
生物化学
重组DNA
基因
毕赤酵母
作者
Michal Avital‐Shmilovici,Doron Shabat
标识
DOI:10.1016/j.bmc.2010.02.038
摘要
Signal amplification dramatically increases the sensitivity of diagnostic methods. Recently, we introduced a new technique for signal amplification that uses a distinctive dendritic chain reaction (DCR) to generate exponential evolution of a diagnostic signal. In this report, we demonstrate how the modular design of our DCR probe can be used to improve the detection sensitivity. We synthesized a new probe based on a methyl carbonate linkage, which has superior stability in aqueous media. Triggered release of methanol, which was oxidized by alcohol oxidase present in the solution, produced hydrogen peroxide that used as a reagent in the DCR amplification technique. The new probe exhibited higher sensitivity in detection of hydrogen peroxide than our previously reported probe.
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