雷苏林
流式细胞术
福尔马赞
活力测定
印版阅读器
细胞生长
恶嗪类
细胞计数
化学
生物测定
细胞
细胞培养
分子生物学
细胞仪
生物
生物化学
荧光
细胞周期
遗传学
物理
有机化学
量子力学
作者
Terry Riss,Richard A. Moravec,Andrew L. Niles,Sarah Duellman,Hélène A Benink,Tracy J Worzella,Lisa Minor
摘要
This chapter is an introductory overview of the most commonly used assay methods to estimate the number of viable cells in multi-well plates. This chapter describes assays where data are recorded using a plate-reader; it does not cover assay methods designed for flow cytometry or high content imaging. The assay methods covered include the use of different classes of colorimetric tetrazolium reagents, resazurin reduction and protease substrates generating a fluorescent signal, the luminogenic ATP assay, and a novel real-time assay to monitor live cells for days in culture. The assays described are based on measurement of a marker activity associated with viable cell number. These assays are used for measuring the results of cell proliferation, testing for cytotoxic effects of compounds, and for multiplexing as an internal control to determine viable cell number during other cell-based assays.
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