生物
生物安全
基因组
载体(分子生物学)
植物病毒
基因组编辑
计算生物学
基因组工程
病毒载体
病毒学
DNA
基因沉默
遗传学
病毒
生物技术
重组DNA
基因
作者
Xiaoyun Wu,Ying Zhang,Xue Jiang,Tingshuai Ma,Yating Guo,Xiaoyun Wu,Yushuang Guo,Xiaofei Cheng
出处
期刊:Virology
[Elsevier]
日期:2024-01-01
卷期号:589: 109922-109922
标识
DOI:10.1016/j.virol.2023.109922
摘要
Plant viruses have been engineered to express proteins and induce gene silencing for decades. Recently, plant viruses have also been used to deliver components into plant cells for genome editing, a technique called virus-induced genome editing (VIGE). Although more than a dozen plant viruses have been engineered into VIGE vectors and VIGE has been successfully accomplished in some plant species, application of VIGE to crops that are difficult to tissue culture and/or have low regeneration efficiency is still tough. This paper discusses factors to consider for an ideal VIGE vector, including insertion capacity for foreign DNA, vertical transmission ability, expression level of the target gene, stability of foreign DNA insertion, and biosafety. We also proposed a step-by-step schedule for excavating the suitable viral vector for VIGE.
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