亲和素
等温滴定量热法
生物素化
化学
生物素
焦磷酸盐
结晶学
生物物理学
立体化学
生物化学
生物
酶
作者
Angelo Spinello,Fabio Lapenta,Matteo De March
出处
期刊:Proteins
[Wiley]
日期:2023-06-15
卷期号:91 (10): 1437-1443
被引量:1
摘要
Abstract The interaction between avidin and its counterpart biotin is one of central importance in biology and has been reproposed and studied at length. However, the binding pocket of avidin is prone to promiscuous binding, able to accommodate even non‐biotinylated ligands. Comprehending the factors that distinguish the extremely strong interaction with biotin to other ligands is an important step to fully picture the thermodynamics of these low‐affinity complexes. Here, we present the complex between chicken white egg avidin and theophylline (TEP), the xanthine derivative used in the therapy of asthma. In the crystal structure, TEP lies in the biotin‐binding pocket with the same orientation and planarity of the aromatic ring of 8‐oxodeoxyguanosine. Indeed, its affinity for avidin measured by isothermal titration calorimetry is in the same μM range as those obtained for the previously characterized nucleoside derivatives. By the use of molecular dynamic simulations, we have investigated the most important intermolecular interactions occurring in the avidin‐TEP binding pocket and compared them with those obtained for the avidin 8‐oxodeoxyguanosine and avidin‐biotin complexes. These results testify the capability of avidin to complex purely aromatic molecules.
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