Objective: To explore the effectiveness of humanistic care in pre-hospital emergency care. Methods: From April 2020 to January 2021, 80 pre-hospital emergency patients were studied. The patients were randomly divided into two groups: a control group (n = 40), which received conventional care, and an experimental group (n = 40), which received humanistic care. The effects of nursing care and psychological state were compared between the two groups. Results: The experimental group showed better nursing outcomes and a more positive psychological state compared to the control group (P < 0.05). Conclusion: Humanistic care in pre-hospital emergency settings is more effective in reducing patients’ anxiety and depression, enhancing the operational abilities and service attitudes of nursing staff, and increasing the emergency success rate.