Transmembrane DNA Sequence Signaling via Hybridization

This study explored a novel transmembrane signaling mechanism using DNA hybridization to facilitate molecular communication across lipid membranes. We designed a system where single-stranded DNA (ssDNA) with cholesterol groups, termed “Driver,” anchors to giant unilamellar vesicles (GUVs). The Driver hybridizes with a complementary ssDNA, “Packet,” carrying signaling sequences. This hybridization enables the transport of DNA sequences across the lipid membrane, triggering internal fluorescence signaling. We demonstrated that this system, named “Chabashira,” effectively facilitates DNA sequence transfer and information processing within GUVs. Our findings suggest potential applications in artificial molecular systems and DNA-based information processing, highlighting the capability for complex and compartmentalized molecular communication.