In the current conditions of development of the agricultural sector of Ukraine, especially in the context of post-war recovery, the relevance of implementing highly efficient technologies for growing vegetable crops in closed soil is increasing. One of the key factors in successful greenhouse production is the rational use of artificial lighting, in particular LED phytolamps with variable spectral composition. The use of modern LED light sources allows increasing the efficiency of photosynthetic processes, activate plant growth and development, reduce energy consumption, and increase the profitability of greenhouse farming. The aim of the article is to experimentally study the effect of the spectral composition of LED phytolamps (red, blue, IR, UV components) on the growth, physiological state, and yield of tomatoes and lettuce grown in protected soil. The paper presents results of the comparison of four spectral lighting options using similar microclimate conditions, which allowed us to clearly trace the effect of each spectrum and their combinations. Data are provided on changes in plant height, leaf surface area, chlorophyll content, biomass mass, and yield for each option. It has been shown for the first time that the combined use of red, blue, infrared, and ultraviolet radiation creates a synergistic effect that ensures balanced development of both vegetative and generative organs of vegetable crops. Statistical analysis of the results confirmed the reliability of the positive impact of the combined spectrum on key agronomic indicators. The presented results can be used to improve methods for selecting LED lighting, designing adaptive spectrum management systems in greenhouses, and as a basis for further scientific research in the field of energy-efficient agricultural production.